Journal: Journal of Clinical Microbiology

Article Title: MGIT Enriched Shotgun Metagenomics for Routine Identification of Nontuberculous Mycobacteria: a Route to Personalized Health Care

doi: 10.1128/jcm.01318-22

Figure Lengend Snippet: M. avium complex phylogeny and M. avium in more detail. The phylogeny illustrates that the NGS-WG typing is perfectly able to identify the species of MAC. However, it has some trouble in determining the M. avium subspecies. This is mainly because the phylogeny shows multiple small subclusters of M. avium . fastBAPS cluster 3 (on level 2) shows distinct Mycobacterium avium subsp. paratuberculosis cluster. fastBAPS cluster 4 (on level 2) shows a distinct M. avium 104-like cluster. CF, cystic fibrosis; TB, tuberculosis; QC, quality control; nod/bronch, nodule/bronchiole; HIV, human immunodeficiency viruses.

Article Snippet: Stored Mycobacterium species were cultured in Middlebrook 7H9 broth with 10% OADC supplement before pellet collection.

Techniques: Control

Journal: Journal of Clinical Microbiology

Article Title: MGIT Enriched Shotgun Metagenomics for Routine Identification of Nontuberculous Mycobacteria: a Route to Personalized Health Care

doi: 10.1128/jcm.01318-22

Figure Lengend Snippet: Flowchart of routine use of MGIT enriched shotgun metagenomics for identification of nontuberculous mycobacteria. All the steps are shown in the flowchart and projected onto our weekly planning. As this workflow is repeated weekly. the maximum time from positive MGIT to DNA isolation is 7 days, meaning the TAT is 4 to 11 days. Icons are obtained from flaticon and are free to use.

Article Snippet: Stored Mycobacterium species were cultured in Middlebrook 7H9 broth with 10% OADC supplement before pellet collection.

Techniques: DNA Extraction

Journal: Current HIV/AIDS reports

Article Title: The Impact of Treatment as Prevention on the HIV Epidemic in British Columbia, Canada

doi: 10.1007/s11904-020-00482-6

Figure Lengend Snippet: List of AIDS-defining illnesses used in this study

Article Snippet: Mycobacterium other or unspecified species disseminated or extrapulmonary , MYCO.

Techniques: Infection

Journal: BMC Microbiology

Article Title: Detection of clinically important non tuberculous mycobacteria (NTM) from pulmonary samples through one-step multiplex PCR assay

doi: 10.1186/s12866-020-01952-y

Figure Lengend Snippet: Amplified PCR product. Image showing genus specific ( 16S rRNA) bands M: Marker 100 bp; Lane1; Mycobacterium tuberculosis (H37Rv), Lane 2; Mycobacterium fortuitum ; Lane 3, 4, 5 & 6: clinically confirmed MTBC; NC: negative control

Article Snippet: Hu C, et al. , [ ] observed five different species of NTM causing pulmonary disease with the help of Mycobacterium Species Identification kit (PCR-reverse dot blot) of DaAn Gene company, such as Mycobacterium intracellulare (70.1%), Mycobacterium abscessus (11.5%) and Mycobacterium avium (11.5%) of isolates.

Techniques: Amplification, Marker, Negative Control

Journal: BMC Microbiology

Article Title: Detection of clinically important non tuberculous mycobacteria (NTM) from pulmonary samples through one-step multiplex PCR assay

doi: 10.1186/s12866-020-01952-y

Figure Lengend Snippet: Oligonucleotide used as primer for amplification

Article Snippet: Hu C, et al. , [ ] observed five different species of NTM causing pulmonary disease with the help of Mycobacterium Species Identification kit (PCR-reverse dot blot) of DaAn Gene company, such as Mycobacterium intracellulare (70.1%), Mycobacterium abscessus (11.5%) and Mycobacterium avium (11.5%) of isolates.

Techniques:

Journal: Heliyon

Article Title: Facile green synthesis of silver nanoparticles derived from the medicinal plant Clerodendrum serratum and its biological activity against Mycobacterium species

doi: 10.1016/j.heliyon.2024.e31116

Figure Lengend Snippet: (a) The figure shows the zone of inhibition of PEG-AgNPs, AgNPs, PLE, and AgNO 3 with Linezolid taken as a standard drug against the three Mycobacterium species i.e., (1) M. smegmatis (2) M. fortuitum, and (3) M. marinum. (b) Hemolytic activity of PEG-AgNPs (100 μg/mL), AgNPs (100 μg/mL), PLE (500 mg/mL), Linezolid (100 μg/mL), AgNO 3 (1 mM), and Triton X-100 (0.5 % v/v) on human blood agar. Triton X-100 and Linezolid were taken as positive control. (c) Flow cytometric analysis to determine the membrane damage of (1) M. smegmatis (2) M. fortuitum, and (3) M. marinum . (i–x) PI staining of all three Mycobacterium species (i) control without PI treatment, (ii) control with PI treatment, (iii) Linezolid, (iv) PEG-AgNPs (1X), (v) PEG-AgNPs (2X), (vi) Plant leaves extract, (vii) AgNPs (1X), (viii) AgNPs (2X), (ix) AgNO 3 , and (x) Triton X-100. Linezolid and Triton X-100 were taken as positive controls. In each quadrant, Q 1 and Q 3 depict the percentage of live cells whereas Q 2 and Q 4 depict the percentage of dead cells. (d) Bar diagram representing the percentage of dead cells of Mycobacterium species treated with test compounds.

Article Snippet: The chemicals used for the culture of Mycobacterium species and the anti-mycobacterial activities were purchased from Himedia and were of molecular biology grade (MB).

Techniques: Inhibition, Activity Assay, Positive Control, Membrane, Staining

Journal: Heliyon

Article Title: Facile green synthesis of silver nanoparticles derived from the medicinal plant Clerodendrum serratum and its biological activity against Mycobacterium species

doi: 10.1016/j.heliyon.2024.e31116

Figure Lengend Snippet: Zone of inhibition and the MIC values of PEG-AgNPs, AgNPs, and PLE against Mycobacterium species.

Article Snippet: The chemicals used for the culture of Mycobacterium species and the anti-mycobacterial activities were purchased from Himedia and were of molecular biology grade (MB).

Techniques: Inhibition, Concentration Assay

Journal: Heliyon

Article Title: Facile green synthesis of silver nanoparticles derived from the medicinal plant Clerodendrum serratum and its biological activity against Mycobacterium species

doi: 10.1016/j.heliyon.2024.e31116

Figure Lengend Snippet: Topographic statistics of biofilm surfaces analyzed through AFM.

Article Snippet: The chemicals used for the culture of Mycobacterium species and the anti-mycobacterial activities were purchased from Himedia and were of molecular biology grade (MB).

Techniques: